REVIEW ARTICLE


A Disposable Electrochemical Glucose Sensor Using Catalytic Subunit of Novel Thermostable Glucose Dehydrogenase



Hideaki Yamaoka, Koji Sode*
Department of Biotechnology, Graduate School of Engineering Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.


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Creative Commons License
© 2008 Yamaoka and Sode

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Biotechnology, Graduate School of Engineering Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan; E-mail: sode@cc.tuat.ac.jp


Abstract

We report here the development of a novel disposable-type glucose enzyme sensor based on a screen-printed carbon electrode (SPCE) employing a catalytic subunit of FAD-dependent glucose dehydrogenase (FADGDH) and a Rucomplex (hexaammineruthenium (III) chloride) as the electron mediator. The whole blood glucose measurement was achieved in 2 seconds using a 500 nl sample of the range of 100-800 mg/ml.

Keywords: Self monitoring of blood glucose, glucose sensor, glucose dehydrogenase.