RESEARCH ARTICLE
Indirect Labeling of Antibodies as a Universal Approach to Increase Sensitivity of Lateral Flow Tests: A Case Study for Mycotoxins Detection
Alexandr E. Urusov1, Alina V. Petrakova1, Anatoly V. Zherdev1, Elena A. Zvereva1, *
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
Issue: Suppl-1, M2
First Page: 113
Last Page: 121
Publisher ID: TOBIOTJ-13-113
DOI: 10.2174/187407070190130113
Article History:
Received Date: 05/04/2019Revision Received Date: 15/07/2019
Acceptance Date: 27/09/2019
Electronic publication date: 15/11/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Objective:
The study aimed at increasing the sensitivity of immunochromatographic tests for the control of toxic contaminants (on the examples of aflatoxin B1 and T-2 toxin) in agricultural products.
Methods:
For reliable immunochromatographic detection of low concentrations of analytes, a replacement of the (specific antibodies – gold nanoparticle) conjugate by a combination of native specific antibodies and anti-species antibodies conjugated with gold nanoparticles was proposed. Different variants of test systems based on the principle of indirect labeling were realized and compared.
Results:
Immunochromatographic assays with indirect labeling for aflatoxin B1 and T-2 toxin were implemented experimentally. A reduction in the detection limit by one to two orders of magnitude was demonstrated.
Conclusion:
The presented results confirm that indirect labeling of specific antibodies overcomes the limitations of the competitive immunochromatographic analysis and can be used to detect analytes of different chemical nature.
