REVIEW ARTICLE


Hydroxyapatite- and Amelogenin Protein-Induced Expression of Mineralization-Related Genes in a Dental Epithelial Cell Line



Liming Xu1, *, Hidemitsu Harada2, Toshiyuki Ikoma3, Akiyoshi Taniguchi1
1 Cell-Sensing Group
2 Department of Oral Anatomy II, Iwate Medical University, School of Dentistry, 1-3-27 Chuodori, Morioka, Iwate 020-8505, Japan
3 Biosystem and Biomolecule Control Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan


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Creative Commons License
© 2008 Xu et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Cell-Sensing, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; E-mail: XU.Liming@nims.go.jp


Abstract

Hydroxyapatite (HAp), a major inorganic component of hard tissues, has been widely used as a novel scaffold for bone or tooth tissue regeneration. However, the effects of HAp on dental cells at the molecular level are poorly understood. In the present study, we evaluated the effects of HAp on differentiation- and mineralization-related gene expression in the dental epithelial cell line (HAT-7). HAT-7 cells were observed to spread on the surface of HAp sintered disks and to increase the expression of several differentiation- and mineralization-related genes. Furthermore, amelogenin and HAp synergistically induced differentiation resulting in increased amelogenin mRNA expression in HAT-7 cells. The results from this study provide important information to develop novel biomaterials for enamel regeneration.