REVIEW ARTICLE
Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension
Alejandro Negrete1, 2, *, Tau Chuan Ling1, 3, Andrew Lyddiatt1, 4
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 29
Last Page: 35
Publisher ID: TOBIOTJ-2-29
DOI: 10.2174/1874070700802010029
Article History:
Received Date: 03/10/2007Revision Received Date: 11/12/2007
Acceptance Date: 11/12/2007
Electronic publication date: 6/2/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium.