REVIEW ARTICLE


Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension



Alejandro Negrete1, 2, *, Tau Chuan Ling1, 3, Andrew Lyddiatt1, 4
1 Biochemical Recovery Group, Department of Chemical Engineering, School of Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
2 Current address: US National Institutes of Health, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA
3 Current address: Department of Process and Food Engineering, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia and
4 Current address: Millipore Biopharmaceutical Division, Bioprocessing Ltd, Medomsley Road, Consett DH8 6SZ, UK


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 1753
Abstract HTML Views: 1843
PDF Downloads: 3374
Total Views/Downloads: 6970
Unique Statistics:

Full-Text HTML Views: 750
Abstract HTML Views: 1072
PDF Downloads: 2350
Total Views/Downloads: 4172



Creative Commons License
© 2008 Negrete et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the National Institutes of Health, National Heart, Lung, and Blood Institute, 9000 Rockville Pike Bldg 10, Rm 7D04, Bethesda, MD 20892, USA; Tel: +1-301-496-6104; Fax: +1-301-496-9985; E-mail: negretea@nhlbi.nih.gov


Abstract

A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium.

Keywords: Adenoviral vectors, serum-free medium, 293 cells, cell culture, suspension.