RESEARCH ARTICLE
Bacillus licheniformis Bearing a High Cellulose-Degrading Activity, which was Isolated as a Heat-Resistant and Micro-Aerophilic Microorganism from Bovine Rumen
Naoko Fujimoto1, Tomoyuki Kosaka2, Toshihiko Nakao3, Mamoru Yamada*, 1, 2
Article Information
Identifiers and Pagination:
Year: 2011Volume: 5
First Page: 7
Last Page: 13
Publisher ID: TOBIOTJ-5-7
DOI: 10.2174/1874070701105010007
Article History:
Received Date: 28/01/2010Revision Received Date: 04/04/2011
Acceptance Date: 12/04/2011
Electronic publication date: 15/6/2011
Collection year: 2011
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Screening of bovine rumen contents for heat-resistant and micro-aerophilic cellulose-degrading microorganisms was performed on carboxymethyl cellulose (CMC) plates at 37 °C-45 °C, and many isolates were found to produce reducing sugars under a static condition. Of those isolates, R8 and R15, which efficiently produced reducing sugars from CMC at a high temperature, were further examined. Taxonomic analysis classified both strains into Bacillus licheniformis. Comparison with B. licheniformis NBRC12200 and Trichoderma reesei NBRC31329 as a type strain under various conditions revealed that R8 and R15 had filter paper- and CMC-degrading abilities, which are absent in the B. licheniformis type strain, and that both isolates produced reducing sugars from CMC more efficiently than did T. reesei type strain at a high temperature under a static condition. These results suggest that R8 and R15 have a strong cellulose-degrading ability at a relatively high temperature under a micro-aerophilic condition.
