RESEARCH ARTICLE


New Test-system Based on the Evaluation of Yeast Cells Resistance to Dehydration-rehydration Stress



Diana Borovikova1, *, Indrikis Muiznieks2, Alexander Rapoport1
1 Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Latvia
2 Department of Microbiology and Biotechnology, Faculty of Biology, University of Latvia, Latvia


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Creative Commons License
© 2015 Borovikova et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Laboratory of Cell Biology, Institute of Microbiology and Biotechnology, University of Latvia, Kronvald blvd., 4, Riga LV-1586, Latvia; Tel: +371 26591328; Fax: +371 67034885; E-mail: borovikovadiana@inbox.lv


Abstract

A new test-system was developed for rapid evaluation of the cytoprotective potential of substances which can be included in cosmetics and health care compositions. It was evaluated by changes of the viability of yeast cells after dehydration/rehydration (D/R) treatment. To check the efficiency of proposed test system we studied the effects of water extracts of the natural peloid, which are used in balneotherapy procedures. It was revealed that the resistance to D/R of cells incubated in peloid water extracts (PWE) was significantly increased (by 30-38%) already after their short incubation in the solutions with low concentrations of the PWE. Meanwhile the membrane permeability was significantly decreased after incubation with PWE. The viability of yeast cells which were more sensitive to D/R stress was increased by PWE to larger extent than the viability of the cells that were initially more resistant to this stress. The positive effect PWE upon yeast cell viability after D/R treatment can be linked with the stabilisation of the cells’ plasma membrane. The results obtained in this study indicate that this new test-system can be recommended as fast, simple and cost-efficient assay for the evaluation of various substances effects upon eukaryotic cells including also the ingredients for cosmetic products.

Keywords: Cell viability, dehydration/rehydration, peloid, permeability, plasma membrane, stress resistance.