1 Institute of Dentistry, Università Cattolica del Sacro Cuore, Largo Francesco Vito - 00168 Rome , Italy
2 Department of Neurosciences, Reproductive and Odontostomatological Sciences, Università di Napoli Federico II, via S. Pansini, 5-80131Naples, Italy
3 Institute of Biochemistry and Clinical Biochemistry, Università Cattolica del Sacro Cuore, Largo Francesco Vito - 00168 Rome , Italy
4 Department of Internal Medicine, Università Cattolica del Sacro Cuore, Largo Francesco Vito - 00168 Rome , Italy
5 Istituto di Chimica del Riconoscimento Molecolare, CNR, Largo Francesco Vito - 00168 Rome , Italy
Background and Objective:
Metabolic syndrome (MetS) is associated with an increased risk of periodontitis even if the mechanism is unknown. Since both MetS and periodontitis are characterized by an alteration of inflammation status, the aim of this pilot study was to determine if differences in ROS metabolism of phagocytes isolated from (A) patients with MetS, (B) patients with both MetS and mild periodontitis, (C) healthy subjects and (D) normal weight subjects with mild periodontitis, were present.
ROS metabolism was studied by a Chemiluminescence (CL) technique: the system was made up of luminol (100 nmol/L) and cells (1 × 105) in the presence or absence of stimulus constituted by opsonized zymosan (0.5 mg). The final volume (1.0 mL) was obtained using modified KRP buffer. ROS production was measured at 25°C for 2 h, using an LB 953 luminometer (Berthold, EG & G Co, Germany). All the experiments were performed in triplicate.
All results are mean ± standard deviation (SD). The group of means was compared by the analysis of variance "(ANOVA)". A value of p < 0.05 was considered significant.
Results showed that basal ROS production (both from PMNs and from PBMs) of groups A, B and D was increased with respect to that obtained from group C (p <0.05).
These results are congruent with literature data, although the actual clinical relevance of the phenomenon remains to be evaluated.
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* Address correspondence to this author at the Department of Neurosciences, Reproductive and Odontostomatological Sciences, Università di Napoli Federico II, Via S. Pansini, 5-80131 Naples, Italy; Tel: +390817462092; E-mail: firstname.lastname@example.org