Antibacterial Activity of Phytochemical Extracts and Endophytic Fungi of Carapa Guianensis Against Enterococcus Faecalis in Endodontic Infections An In Vitro Study
Maria do Carmo M. de Miranda1, Clarice M. Carvalho2, Fernando S.E.D.V. Faria2, Antonio M-S. Nobreza3, Thiago M. Pereira3, Keliin Pivatto3, Marcus V.C. da Costa4, Orlando A. Guedes3, Cyntia R. de Araújo Estrela3, Álvaro H. Borges3, *
1 Institute of Biodiversity and Biotechnology of the Legal Amazon, Federal University of Acre, Rio Branco, Acre, Brazil
2 Center for Biological and Nature Sciences, Federal University of Acre, Rio Branco, Acre, Brazil
3 Department of Oral Sciences, University of Cuiaba, Cuiaba, Mato Grosso, Brazil
4 Faculty of Medicine, Federal University of Goias, Goiânia, Goiás, Brazil
The objective of this study was to evaluate the antibacterial activity of phytochemical extracts and endophytic fungi of Carapa guianensis against Enterococcus faecalis. Carapa guianensis leaves and stems were collected to obtain phytochemical extracts and fungal metabolites and evaluated for in vitro antibacterial activity against E. faecalis using the disc diffusion method and dentin blocks with bacterial biofilm.
Thirty dentin blocks were prepared and contaminated for 60 days with E. faecalis. The specimens were randomly divided into 6 experimental groups according to the test solution used: G1 – hexane stem extract of Carapa guianensis; G2 – methanol stem extract of Carapa guianensis; G3 – methanol leaf extract of Carapa guianensis; G4 – ethyl acetate extract of the endophytic fungus Penicillium isolated from Carapa guianensis; G5 – negative control, with no addition of bacterial inoculum; G6 – positive control.
Bacterial growth was analyzed by spectrophotometry after 14 days of direct contact between the extracts and dentin blocks. The hexane-stem, methanol-stem, methanol-leaf, and ethyl-acetate endophytic fungus Penicillium extracts inhibited bacterial growth in 100% of the samples.
The present study demonstrated the antibacterial potential of phytochemical extracts and endophytic fungi of Carapa guianensis against E. faecalis.
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* Address correspondence to this author at the Department of Oral Sciences, School of Dentistry, University of Cuiabá, Avenida Manoel José de Arruda, 3100, Jardim Europa, 78065-900, Cuiabá, MT, Brazil; Tel: +55 65 3363-1264;