RESEARCH ARTICLE


Frequency of Iron Uptake Proteins Related Genes Among Klebsiella pneumoniae Isolates



Tahereh Elhaki1, 2
iD
, Ali Gheysarzadeh2
iD
, Nourkhoda Sadeghifard1, 2, *
iD
, Iraj Pakzad1, 2
iD
, Ava Behrouzi3
iD
, Morovat Taherikalani2
iD
, Farid A. Jalilian2
iD
, Mohsen Tabasi4
iD
, Reza Azizian1, 2
iD

1 Department of Medical Microbiology, Medicine Faculty, Ilam University of Medical Science, Iran
2 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
3 Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
4 Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran


Article Metrics

CrossRef Citations:
1
Total Statistics:

Full-Text HTML Views: 2719
Abstract HTML Views: 1234
PDF Downloads: 643
ePub Downloads: 350
Total Views/Downloads: 4946
Unique Statistics:

Full-Text HTML Views: 1201
Abstract HTML Views: 562
PDF Downloads: 433
ePub Downloads: 225
Total Views/Downloads: 2421



Creative Commons License
© 2020 Elhaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. Tel: +98-8412227101, Fax: +98-8412227136, E-mail: sadeghifard@gmail.com


Abstract

Aims:

The present study aimed to evaluate Iron uptake protein-related genes in clinical and environmental Klebsiella pneumoniae isolates.

Background:

Klebsiella pneumoniae as an opportunistic pathogen cause infections in immunocompromised patients. Iron uptake systems play an important role in the pathogenesis of Klebsiella pneumonia.

Objectives:

This study was designed to investigate the prevalence of iron uptake coding genes among isolates of Klebsiella pneumonia.

Materials and Methods:

A total of 300 isolates of Klebsiella pneumonia including 150 clinical isolates and 150 environmental isolates were selected. Finally, the frequency of iroN, iucD, kfuA,hmuR, and ybt [yHPI] genes were detected by PCR method.

Results:

The frequency of kfuA, iucD, iroN, yHPI in clinical isolates were 33.3%, 16.7%, 24.7%, 15.3%, respectively and these genes among environmental isolates were 20.7%, 6%, 49.3% and 0.7%, respectively. Among the clinical isolates, the most frequency genes were kfuA gene [50 isolates] and after that iroN [37 isolates], iucD [25 isolates] and yHPI [23 isolates], the genes with the most frequency among environmental isolates were iroN gene [74 isolates] and following that kfuA [31 isolates], iucD [9 isolates] and yHPI [1 isolate]. No hmuR positive samples among all clinical or environmental isolates were found.

Conclusion:

The result of this study showed that because of the high frequency of ferric iron system coding gene kfu among clinical isolates, this system might play an important role in the survival of bacteria against its host.

Keywords: : Klebsiella pneumoniae, Iron uptake gene, IroN, Iucd, Kfua, Hmur, Aybt, PCR.