The Open Orthopaedics Journal

ISSN: 1874-3250 ― Volume 14, 2020

PDGF-BB Induces Formation of Bridging Callus After Reconstructive Surgery of Large Bone Defect

I. Gede Eka Wiratnaya*
Department of Orthopedic and Traumatology, Medical Faculty of Udayana University / Sanglah Hospital, Diponegoro, Denpasar, Bali, Indonesia



Reconstructive surgery by using allografts often conducted to manage large bone defects, either due to traumatic or non-traumatic causes. However, poor vascularization of the graft bed is still problematic. To overcome this, bone tissue engineering method has been developed that uses growth factor as an angiogenic stimulator, such as platelet derived growth factor BB (PDGF BB).


This study aimed to evaluate the administration of recombinant rat Platelet Derived Growth Factor BB (rrPDGF BB) on bone healing process, showed by formation of bridging callus, Vascular Endothelial Growth Factor (VEGF), Bone Morphogenetic Protein-2 (BMP-2) and osteocalcin in massive fresh frozen allograft post reconstructive surgery.


This was a Post Test Only Control Group Design study involved 32 Wistar rats divided into two groups, i.e. treatment group (defect on right femoral bone and received fresh frozen allograft with the addition of rrPDGF BB) and control group (without addition of rrPDGF BB). Expression of VEGF, BMP-2 and osteocalcin was identified through immunohistochemistry.


A significantly higher expression of VEGF, BMP-2 and osteocalcin was observed in the treatment group as compared to the control group (p < 0.05). The presence of bridging callus on the fresh frozen allograft also showed to be significant (p = 0.003). Path analysis showed formation of bridging callus after administration of PDGF on allograft occur through three pathways, in which VEGF holds the most important role.


The application of rrPDGF BB significantly enhances the formation of new bone through increased expression of VEGF, BMP-2 and osteocalcin.

Keywords: Bridging callus, BMP-2, PDGF BB, VEGF, Osteocalcin, Traumatic.

Article Information

Identifiers and Pagination:

Year: 2018
Volume: 12
First Page: 583
Last Page: 594
Publisher Id: TOORTHJ-12-583
DOI: 10.2174/1874325001812010583

Article History:

Received Date: 31/8/2018
Revision Received Date: 14/11/2018
Acceptance Date: 20/11/2018
Electronic publication date: 31/12/2018
Collection year: 2018

© 2018 I Gede Eka Wiratnaya.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Orthopedic and Traumatology, Medical Faculty of Udayana University / Sanglah Hospital, Diponegoro, Denpasar, Bali, Indonesia; Tel: +62 813 3849 3832; E-mail:

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