The Open Orthopaedics Journal




ISSN: 1874-3250 ― Volume 13, 2019

Osteocompatibility of Biofilm Inhibitors



Monica Rawson, Warren Haggard , Jessica A Jennings*
Biomedical Engineering, University of Memphis, 330 Engineering Technology Building, Memphis, TN 38152, USA

Abstract

The demand for infection prevention therapies has led to the discovery of several biofilm inhibitors. These inhibiting signals are released by bacteria, fungi, or marine organisms to signal biofilm dispersal or disruption in Gram-positive, Gram-negative, and fungal microorganisms. The purpose of this study was to test the biocompatibility of five different naturally-produced biofilm chemical dispersal and inhibition signals with osteoblast-like cells: D-amino acids (D-AA), lysostaphin (LS), farnesol, cis-2-decenoic acid (C2DA), and desformyl flustrabromine (dFBr). In this preliminary study, compatibility of these anti-biofilm agents with differentiating osteoblasts was examined over a 21 days period at levels above and below concentrations active against bacterial biofilm. Anti-biofilm compounds listed above were serially diluted in osteogenic media and added to cultures of MC3T3 cells. Cell viability and cytotoxicity, after exposure to each anti-biofilm agent, were measured using a DNA assay. Differentiation characteristics of osteoblasts were determined qualitatively by observing staining of mineral deposits and quantitatively with an alkaline phosphatase assay. D-AA, LS, and C2DA were all biocompatible within the reported biofilm inhibitory concentration ranges and supported osteoblast differentiation. Farnesol and dFBr induced cytotoxic responses within the reported biofilm inhibitory concentration range and low doses of dFBr were found to inhibit osteoblast differentiation. At high concentrations, such as those that may be present after local delivery, many of these biofilm inhibitors can have effects on cellular viability and osteoblast function. Concentrations at which negative effects on osteoblasts occur should serve as upper limits for delivery to orthopaedic trauma sites and guide development of these potential therapeutics for orthopaedics.

Keywords: Biocompatibility, biofilm, dispersal agents, infection, mineralization, osteoblast..


Article Information


Identifiers and Pagination:

Year: 2014
Volume: 8
First Page: 442
Last Page: 449
Publisher Id: TOORTHJ-8-442
DOI: 10.2174/1874325001408010442

Article History:

Received Date: 26/6/2014
Revision Received Date: 1/10/2014
Acceptance Date: 19/10/2014
Electronic publication date: 26 /11/2014
Collection year: 2014

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© Rawson et al.; Licensee Bentham Open.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/) which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.


* Address correspondence to this author at the Biomedical Engineering, University of Memphis, 330 Engineering Technology Building, Memphis, TN 38152, USA; Tel: (901)678-3733; Fax: (901)678-5281; E-mail: jjnnings@memphis.edu



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