ELISA | AlphaLISA | |
---|---|---|
Selectivity | High | High |
Sensitivity | Sensitive (pM range) | Sensitive (sub-pM) |
Assay Format | Heterogeneous | Homogenous |
Antibody req. | Requires matched antibody pairs | Used with antibody pairs |
Assay vol. | large (25-50 µl) | small (≥ 5 µl) |
Dynamic range | limited (~2 orders of mag.) | large (~4 orders of mag.) |
Instrumentation | any luminescent reader | requires high energy laser |