Table 1: Activities of Confirmed Inhibitors from Chemical Library Using Serum-Based and Purified PON1 Assays

Compound Asinex Identifier a Structure Serum PON1 IC50 (µM) b Purified PON1
% Inhibition c IC50 (µM) d
2HQ N/A 7.9 84 4.4
1 ASN 08910296 8.5 90 10.6
2 ASN 08912428 18.6 13 n.d. e
3 BAS 09529674 15.3 103 0.76
4 BAS 01369653 10.3 90 5.3
5 BAS 09683917 21.8 -2 n.d.
6 BAS 09683916 26.6 6 n.d.
7 BAS 03551158 12.5 103 0.42
8 ASN 05298740 13.2 12 n.d.
9 BAS 05307909 14.9 70 n.d.
10 BAS 02915353 18.4 19 n.d.
11 BAS 09588626 20.6 92 4.8
12 BAS 01814176 21.1 18 n.d.

a Chemical identifier number provided by Asinex Corporation.
b IC50 value determined by the serum paraoxonase assay.
c Compounds were tested at 20 µM in triplicate determinations for each compound. Data are average % inhibition values from two experiments.
d Data are average IC50 values determined from two independent experiments, except for compounds 1 and 6.
e Not determined.