| Ramm (Ref. #24) | Price (Ref. #25) | Mitchell (Ref. #4) | Radio (Ref. #5) | Yeyeodu (This paper) | |
|---|---|---|---|---|---|
| Detection method(s) | Abs: primary + FITC-secondary | Coomassie blue G-250 | Hoechst + Ab: primary + AlexaFluor + 488-secondary | Hoechst + Ab: primary + AlexaFluor + 488-secondary | a) Hoechst + GFP expressing cells or b) Hoechst + CellMask Red dye |
| Plate/pretreat | 8 h | 5 d | 4-6 d | 1-4 d | 16-24 h |
| Treat | 3 d | 4 d | 16-24 h | 4 d | 2 d |
| Total time in culture | >3 d | 9 d | >4.5 d | 5 d | <3 d |
| Cell preparation for imaging | |||||
| Fix(/stain) | 2 h | 1 h | 30 min | 20 min | 30 min |
| Permeabilize/wash | 30-60 min | - | 30 min | 30 min | - |
| Primary Antibody | 1 h | - | 18 h | 1 h | - |
| Secondary Antibody | 1 h | - | 1 h | 1 h | - |
| Final stain | - | ON | - | - | - |
| Total preparation time | 4.5-5 h | ON | ON | 3 h | 30 min |