Fig. (2) Destabilized luciferase genes increase the reporter response and reduce the assay time to reach maximum induction. luc2, firefly
luciferase; luc2P, firefly luciferase with hPEST sequence; luc2CP, firefly luciferase with hCL1 and hPEST sequences. HEK293 cells transiently
expressing various versions of SRE- or SRF-RE- reporters (luc2, luc2P or luc2CP) were serum starved, induced with 20% FBS plus
10ng/ml PMA for SRE (2B, 2D), or with 20% FBS for SRF-RE (2C, 2E) for 2-8 hours. Cells not induced after serum starvation were used as
control (uninduced). Firefly luciferase activity (RLU) were determined using One-Glo assay system and measured on the GloMax 96 multiplate
luminometer. (2B, 2C) Firefly luciferase activity (RLU) and fold of induction measured after six hours of induction. (2D, 2E) Time
course of fold of induction of SRE- and SRF-RE measured every two hours for 8 hours after induction.