Fig. (3) GPCR assays for G_s, G_i, G_q, and G₁₂ coupled receptors in 384-well format. HEK293 cells used are either stably expressing D₁ dopamine receptor and CRE-luc2P (A), m3 muscarinic receptor with NFAT-RE-luc2P reporter (B), SRF-RE-luc2P reporter (test endogenous EDG receptor) (D), or transiently expressing m₄ muscarinic receptor with SRE-luc2CP reporter (C). Cells were induced with various agonists in 384-well format. Agonists used (time of induction): 1 µM dopamine (4 hours); 1 µM muscarine chloride (8 hours); 10 µM acetylcholine (2 hours); 10 µM LPA (6 hours); Firefly luciferase activity was measured using One-Glo assay system. Fold of induction and Z’ values were determined as described in “Materials and Methodology”.