Table 1:
Apparent Rate Constants (
k
)
a
for Binding Reactions between H272F-based Variants and Haloalkane Ligands as Determined by FP
Variant
k
(TMR-ligand)
k
(FAM-ligand)
HT2
3.0 x 10
6
1.6 x 10
4
HT3
4.0 x 10
6
2.9 x 10
4
HT6
1.1 x 10
7
6.7 x 10
5
HT7
1.9 x 10
7
2.0 x 10
6
a
Rate constants (M
-1
sec-
1
at 25
°
C) were calculated from the second-order rate equation (see reference 24).