Fig. (2) (A) MANT-ADP fluorescence decreased as the ionic strength of the assay solution increased. In the absence of NaCl, 0.01% Triton had no effect on MANT-ADP fluorescence. (B) The assay window increased linearly as concentrations of AMPK, ADP, and MANT-ADP were increased at a constant molar ratio. (A) n = 4 wells per data point; (B) n = 6 wells per data point. Data points are mean ± standard deviation. Z’-factors ≥ 0.6.