Fig. (1) Workflow comparison of the proposed dye-based neurite outgrowth assay with traditional immunocytochemistry. (A) Traditional immunocytochemistry (antibody-based staining) for neurite outgrowth requires numerous pipette steps (arrows) and several hours to process. In contrast, the dye-based staining method in this report uses a streamlined workflow that typically requires approximately 30 minutes to complete. (B) Outline of the neurite outgrowth assay workflow.