Fig. (1) Workflow comparison of the proposed dye-based neurite outgrowth assay with traditional immunocytochemistry. (A) Traditional
immunocytochemistry (antibody-based staining) for neurite outgrowth requires numerous pipette steps (arrows) and several hours to process.
In contrast, the dye-based staining method in this report uses a streamlined workflow that typically requires approximately 30 minutes to
complete. (B) Outline of the neurite outgrowth assay workflow.