Fig. (4) Plate reader quantification of relative neurite outgrowth and cell viability using primary rat cortex neurons. Cryopreserved cells were thawed and plated in 96-well format, cultured for 7 days, and then treated with compounds for an additional 4 days prior to assaying them. Relative cell viability (▼) and neurite outgrowth (■) were measured using a bottom-read fluorescence microplate reader (data plotted are the mean ± SEM of triplicate wells) and representative images were taken of the following conditions: (A) untreated control, or maximum test concentration for (B) CCCP, (C) Nocodazole, (D) SAHA, and (E) Stauorosporine.