Fig. (5) Plate reader quantification of relative neurite outgrowth and cell viability using human iPSC-derived iCell Neurons. Cryopreserved cells were thawed, plated in 96-well format and allowed to recover overnight. The next day, test compounds were either applied once for 1 hour (acute exposure) and then replaced with fresh medium thereafter, or applied and left on (chronic exposure) and replenished during subsequent feedings (every 3 – 4 days). After 11 days in culture, the cells were pre-fixed with 4%formaldehyde, stained, and relative cell viability (▼) and neurite outgrowth (■) were measured using a bottom-read fluorescence microplate reader (data plotted are the mean ± SEM of triplicate wells). Data plots and selected compound-treated images are as follows: (A) untreated control, (B and E) Antimycin A, (C and F) Nocodazole, (D and G) Stauorosporine.