Design of FRET Probes for SNP RS1006737, Related to Mood Disorder

Table 1: Oligonucleotides designed for a nested real time PCR for rs1006737.

Oligo Name	Sequence 5'-3'	Length (nt)	Position NG_008801
OG644F	GGCTTCAGAGTCCACTTGGC	20	270271
OG645R	TGGGCACATTCAAACCTGAA	20	270410
OG646F	CAAAGTCTTGCTATCAATTACATA	24.	270296
OG647R	CTGAGAGACACTGTGAGGTC	20	270380
OG648_Fam	CATTCCATCTCAGCCC^Fam	16	270325
OG649_R640	^Red640AAGTGTTTTCAGAGCC^PHO	16	270342

Note: 644-645 = external PCR, amplicon length = 140 bp
646-647 = internal PCR, amplicon length = 85 bp
Theoretical annealing temp. = 50°C for both reactions.