Fig. (3) Polyacrylamide gel electrophoresis of cysteine protease from Actinidia arguta.Electrophoresis of the purified enzyme was carried out on a Mini-protean TGX precast gel (any kD) in the presence of SDS and the gel was stained with Coomassie Brilliant Blue G-250. Lane 1 contained 5 μg of the purified protein in the presence of β-ME. Precision Plus protein standards (Bio-Rad) were also used for estimation of molecular weight.