Fig. (6) Inhibition of the activity of cysteine protease from Actinidia arguta by several protease inhibitors including E-64, kinin-free low molecular weight kininogen and cysteine C.The purified enzyme (0.5 μg) was pre-incubated with each inhibitor for 10 min at room temperature in 50 mM sodium phosphate buffer, pH 7.0, and assays were started by adding 100 μM Z-Leu-Arg-MCA. The reaction mixtures were incubated for an additional 10 min at 37°C. Each value is the mean ± SE from triplicate experiments.