Fig. (4) Kinetic measurement of eL42:p53 or eL42:Mdm2 interactions.The His-tagged recombinant human eL42 was immobilized on the surface of CM5 sensor chip at low density resonance units (RU). (A): kinetics of interaction between eL42 and p53 at the concentrations of 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5 and 4 nM. (B): the half maximal effective concentration (EC₅₀) of p53 corresponding to half-maximal saturation of eL42 was estimated to be 2.2 x 10^-9 M. (C): kinetics of interaction between eL42 and Mdm2 at the concentrations of 0, 20, 40, 50, 60 and 80 nM. (D): specific binding of p53 to eL42 versus negative control (FMTS 500 nM) and specific recognition by the anti-eL42 antibodies on immobilized eL42. (E): demonstration of specific binding of p53 (50 nM) and Mdm2 (50 nM) on eL42 versus negative control (FMTS 500 nM).