Fig. (5) Kinetic measurement of Mdm2:p53.The His-tagged recombinant human Mdm2 was immobilized on the surface of CM5 sensor chip at low density resonance units (RU). (A): kinetics of interaction between Mdm2 and p53 at the concentrations of 0, 2, 4, 6, 8, 10, 12 nM. (B): demonstration of specific binding of p53 at the concentration of 5 nM or 10 nM on Mdm2 versus negative control (FMTS 500nM).