Fig. (6) Interactions between human eL42 and p53 in the absence or in the presence of PLP. (A): Kinetics of PLP on eL42. The His-tagged recombinant human eL42 was immobilized on the surface of CM5 sensor chip at low density resonance units to prevent mass transport (RU). Various concentrations of PLP (0, 100, 200, 300, 400, 500, 600, 700 and 800 μM) were run over the chip surface and BiaEvaluation 4.1 Software was used to evaluate kinetic parameters; (B): comparative binding of p53 (5 nM) or PLP-treated p53 (5 nM) on eL42; (C): Kinetics of PLP-treated p53 on eL42. Various concentrations of PLP-treated p53 (0, 1, 1.5, 2, 2.5, 3, 3.5, 4 and 5 nM) in 1 mM PLP were run over the chip surface. The association constant (ka or kon) and the dissociation constant (kd or koff) obtained were 2.36 x 103 M-1. s-1 and 2.55 x 10-4 s-1 respectively, corresponding to a binding affinity (KD) of 1,08 x 10-7 M. The value of Chi2 was 5.23; (D): EC50 estimation.