Fig. (7) Effect of added recombinant eL42 protein on the activity of human 80S ribosomes. (A): Effect of increasing concentrations of eL42 on the in vitro poly(U)-dependent poly(Phe) synthesis activity of human 80S ribosomes. (B): Kinetics of poly(U)-dependent poly(Phe) synthesis reaction in the absence (circles) or in the presence of human recombinant eL42 at the concentration of 35 μM (triangles). (C): effect of eL42 addition to the incubation mixture of the poly(U)-dependent poly(Phe) synthesis reaction catalyzed by human 80S ribosomes when [14C]Phe incorporation has reached a plateau value prior to the addition of eL42. For details, see Materials and Methods.