| Mutant | Disrupted gene | Source organism | Accession number | Orthologue | Function and description a | Query length | Target length | E-value | Target coverage | Alignment coverage | Identity | Positive |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DG2 | KLMA_20059 | Saccharomyces cerevisiae S288c | NP_011480 | Mig1p | Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion | 556 | 504 | 1.9E-38 | 91% | 41% | 18% | 23% |
| Kluyveromyces lactis NRRL Y-1140 | XP_454446 | Hypothetical protein | Uniprot|P50898 K. lactis MIG1 Regulatory protein MIG1 | 556 | 474 | 5.57E-132 | 85% | 100% | 50% | 62% | ||
| DG4 | KLMA_20787 | Saccharomyces cerevisiae S288c | NP_014872 | Wtm2p | Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats | 455 | 467 | 7.51E-77 | 100% | 100% | 35% | 51% |
| Kluyveromyces lactis NRRL Y-1140 | XP_453386 | Hypothetical protein | Similar to gnl|GLV|CAGL0M02563g Candida glabrata CAGL0M02563g and weakly similar to uniprot|Q12206 YOR229W S. cerevisiae WTM2 | 455 | 450 | 1.7E-170 | 99% | 100% | 56% | 72% | ||
| DG6 | KLMA_60099 | Saccharomyces cerevisiae S288c | NP_014673 | Dfg16p | Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p | 479 | 619 | 4.1E-59 | 100% | 81% | 25% | 44% |
| Kluyveromyces lactis NRRL Y-1140 | XP_454708 | Hypothetical protein | Some similarities with uniprot|Q99234 S. cerevisiae YOR030W DFG16 | 479 | 482 | 1.42E-178 | 100% | 100% | 56% | 74% | ||
| DG9 | KLMA_20447 | Saccharomyces cerevisiae S288c | NP_011977 | Ctm1p | Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth | 511 | 585 | 1.32E-44 | 100% | 100% | 27% | 49% |
| Kluyveromyces lactis NRRL Y-1140 | XP_454919 | Hypothetical protein | Weakly similar to uniprot|P38818 YHR109W S. cerevisiae CTM1 | 511 | 503 | 4.65E-175 | 98% | 99% | 50% | 67% | ||
| DG41 | KLMA_60404 | Saccharomyces cerevisiae S288c | NP_015276 | Dig1p | MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p | 376 | 452 | 4.00E-07 | 100% | 43% | 14% | 23% |
| Kluyveromyces lactis NRRL Y-1140 | XP_453577 | Hypothetical protein | Conserved hypothetical protein | 376 | 372 | 4.3E-132 | 99% | 100% | 58% | 70% | ||
| DG50 | KLMA_40242 | Saccharomyces cerevisiae S288c | NP_010622 | Msn5p | Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm | 1221 | 1224 | 0 | 100% | 100% | 67% | 84% |
| Kluyveromyces lactis NRRL Y-1140 | XP_456046 | Hypothetical protein | Highly similar to uniprot|P52918 S. cerevisiae YDR335W MSN5 | 1221 | 1221 | 0 | 100% | 100% | 91% | 97% | ||
| DG54 | KLMA_60055 | Saccharomyces cerevisiae S288c | NP_011743 | Alg10p | Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 | 509 | 525 | 0 | 100% | 100% | 56% | 73% |
| Kluyveromyces lactis NRRL Y-1140 | XP_454662 | Hypothetical protein | Similar to uniprot|P50076 S. cerevisiae YGR227W DIE2 | 509 | 533 | 0 | 100% | 100% | 80% | 89% | ||
| DG68 | KLMA_10391 | Saccharomyces cerevisiae S288c | NP_012750 | Nnk1p | Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm | 806 | 928 | 8.04E-103 | 100% | 93% | 31% | 46% |
| Kluyveromyces lactis NRRL Y-1140 | XP_451302 | Hypothetical protein | Similar to uniprot|Q757Y0 Ashbya gossypii AEL120W AEL120Wp and some similarities with YKL171W uniprot|P36003 S. cerevisiae YKL171W | 806 | 800 | 0 | 99% | 100% | 69% | 82% | ||
| DG71 | KLMA_60409 | Saccharomyces cerevisiae S288c | NP_011265 | Zip2p | Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress | 678 | 704 | 1.06E-19 | 100% | 100% | 25% | 45% |
| Kluyveromyces lactis NRRL Y-1140 | XP_453570 | Hypothetical protein | Weakly similar to uniprot|P53061 S. cerevisiae YGL249W ZIP2: Required for 'ZIPpering' up meiotic chromosomes during chromosome synapsis involved in meiotic recombination and disjunction | 678 | 675 | 2.29E-157 | 100% | 100% | 40% | 62% | ||
| DG72 | KLMA_70178 | Saccharomyces cerevisiae S288c | NP_010990 | Rgi2p | Hypothetical protein; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress | 161 | 161 | 1.12E-70 | 100% | 99% | 68% | 79% |
| Kluyveromyces lactis NRRL Y-1140 | XP_452559 | Hypothetical protein | Highly similar to uniprot|P40188 S. cerevisiae YIL057C Hypothetical ORF | 161 | 163 | 2.25E-105 | 100% | 100% | 91% | 94% | ||
| DG79 | KLMA_20626 | Saccharomyces cerevisiae S288c | NP_014071 | Mdj2p | Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain | 148 | 146 | 4.81E-48 | 99% | 100% | 49% | 75% |
| Kluyveromyces lactis NRRL Y-1140 | XP_454039 | Hypothetical protein | Similar to uniprot|P42834 S. cerevisiae YNL328C MDJ2: Protein of the mitochondrial inner membrane function partially overlaps that of Mdj1p which is a chaperone involved in folding of mitochondrially synthesized proteins in the mitochondrial matrix member of the DnaJ family | 148 | 146 | 9.36E-78 | 99% | 99% | 76% | 92% | ||
| DG80 | KLMA_30168 | Saccharomyces cerevisiae S288c | NP_012447 | Arg3p | Ornithine carbamoyltransferase (carbamoylphosphate: L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine | 340 | 338 | 0 | 99% | 98% | 72% | 83% |
| Kluyveromyces lactis NRRL Y-1140 | XP_455725 | Hypothetical protein | Similar to uniprot|P05150 YJL088W S. cerevisiae ARG3 | 340 | 334 | 0 | 98% | 94% | 85% | 90% |