Fig. (3) Cardiac developmental gene expression in EB from D3-eGFP and D3G4 line B. (A) Percentage of EB (n=19/cell line) containing microscopically detectable areas of contracting cells on days 9-12 past the onset of differentiation. The time of RNA extraction for (q)RT-PCR analysis is indicated. (B) mRNA levels for the indicated genes as measured by qRT-PCR. Data was normalized to mouse Hprt cDNA and is expressed relative to undifferentiated D3 mESC. (C) qRT-PCR products identified by agarose gel electroporesis and ethidium bromide staining (m, mouse; h, human).