Fig. (4) Cytokine-induced apoptosis of fetal lung epithelial cells is NO
dependent. (A) The cells were pre-incubated with or without 1400W
(25 M) and then incubated with cytokines for 24 h. Total nitrogen
oxides were measured in the media. (B) The cells were preincubated
with or without 1400W (25 µM) for 1 h and followed by incubation
with cytokines for 24 h. Cell death was determined by dual
fluorescence photomicroscopy utilizing Rhodamine 123 and YoPro-1.
The data shown were representative of three experiments (*p<0.05).