Fig. (5) Experimental verification of detected gene network from Cdk7 to EGFR and N-myc1 using a recombinant retrovirus expression system. (A) The protein levels of exogenous Cdk7 (HA), total Cdk7 (Cdk7), EGFR, and N-myc1 were detected by western blotting. Representative blots obtained from two independent samples are shown in the figure. (B) EGFR and N-myc1 protein levels were quantitatively analyzed. Data are the average plus standard deviation of 6 western blots from two independent samples for each group. *, P < 0.05; **, P < 0.01.