Table 3: The Concentrations of Detection Reagents

Fluorochrome Manufacturer Stock Concentration Working Dilution
Avidin-Cy5 BDS, Pittsburgh, PA 1 mg/ml 1.5:500
AMCA-avidin Vector, Burlingame, CA 5 mg/ml 1.0:200
Biotinylated goat-anti-avidin Vector, Burlingame, CA 500 µg/ml 1.0:100
Rhodamine-labeled sheep-anti-digoxigenin Roche Molecular, Indianapolis, IN 200 µg/ml 1.0:100
Mouse-anti-FITC DAKO, Carpinteria, CA 430 µg/ml 1.0:200
FITC-labeled goat-anti-mouse Vector, Burlingame, CA 1.5 mg/ml 1.0:300