Fig. (3) The effect of aldosterone and eplerenone on HBMEC migration. HBMEC (200,000), cultivated in M131 + 10% fetal calf serum, were layered on 0.3% gelatin for 18 h to attain 90% confluence. A fissure was incised by the technique of the Wound-healing test (Mirshahi et al., 2006). The flasks were cultivated in the presence of M 131 + 2% fetal calf serum and the number of cells migrating to the wound edge was quantitated by Archimed program of Microvision 6h, 18h, 27h and 48h later. The experiment was repeated eight times using control cultures, 100 nM aldosterone, 400 nM eplerenone, agonist plus antagonist, as described in the Methods section. After 48 h at 37°C, compared to the control, eplerenone = p<0.003; aldosterone + eplerenone = p<0.003; aldosterone = not significant (NS); aldosterone vs eplerenone = NS; eplerenone vs aldosterone + eplerenone = NS.