Fig. (4) Inhibition of HBMEC proliferation by aldosterone and eplerenone. HBMEC (60,000) were cultivated in M131 + 10% fetal calf serum, detached by acutase, exposed to trypan blue and the surviving cells were counted 0, 18 and 36 h later. The experiment was repeated five times with control cultures, 100 nM aldosterone, 400nM eplerenone, agonist plus antagonist, as detailed in the Methods section. After 36 h, compared to the control, aldosterone = p<0.001; eplerenone = p>0.05; aldosterone + eplerenone = p<0.001; aldosterone vs eplerenone = p<0.003.