Fig. (5) Qualitative analysis of RNA used to assess gene modulation by aldosterone. Total RNA from HBMEC was electrophoresed on agarose (A) to verify the quality of mRNA. Electrophreograms (B) performed on the electrophoresis peaks permitted the calculation of 28S/18S ratio. A value greater than 1.5 indicated qualitative integrity of the RNAs. Here, the ratios were 1.72 for the control and 1.85 for the aldosterone treated group. Furthermore, the samples did not show any additional degradation peaks in the low molecular weight range. These samples were used for linear amplification of 1800 genes that code for cytokines, monokines, growth factors, angiogenic effectors and cell metabolism, normal and malignant growth, as detailed in the Methods section.