Table 1: 16S rDNA Bacterial Primers and Annealing Temperatures Used in Real Time PCR and Expected Product Size

Organism	Sequence	Size (bp)	Annealing Temperature	Ref
Universal 1	GATTAGATACCCTGGTAGTCCAC	733	52	16
	TACCTTGTTACGACTT
Universal 2	TCCTACGGGAGGCAGCAGT	466	58	26
	GGACTACCAGGGTATCTAATCCTGTT
Universal 3	CGCTAGTAATCGTGGATCAGAATG	69	58	35
	TGTGACGGGCGGTGTGTA
Aggregatibacter actinomycetemcomitans	CTAGGTATTGCGAAACAATTTG	262	55	32
	CCTGAAATTAAGCTGGTAATC
Porphyromonas gingivalis	AGGCAGCTTGCCATACTGCG	404	60	8
	ACTGTTAGCAACTACCGATGT
Treponema denticola	TAATACCGAATGTGCTCATTTACAT	316	59	8
	TCAAAGAAGCATTCCCTCTTCTTCTTA
Tannerella forsythia	GCGTATGTAACCTGCCCGCA	641	62	8
	TGCTTCAGTGTCAGTTATACCT
Prevotella intermedia	CGTGGACCAAAGATTCATCGGTGGA	259	64	30
	CCGCTTTACTCCCCAACAAA
Fusobacterium nucleatum	AGAGTTTGATCCTGGCTCAG	360	60	31
	GTCATCGTGCACACAGAATTGCTG
Campylobacter rectus	TTTCGGAGCGTAAACTCCTTTTC	598	60	15
	TTTCTGCAAGCAGACACTCTT