Table 5: qPCR Enumeration of Total Bacteria and Specific Bacterial Species in 100 ┬Ál of Plaque DNA

Total Bacteria (x108) Aa (x104) Pg (x105) Td (x106) Tf (x106) Fn (x106) Pi (x105) Cr (x105) Sum of 7 (x107)
1 5.29 0.00*(0.00%) 0.037(0.00%) 2.02(0.38%) 8.29(1.57%) 32.9(6.21%) 207(3.91%) 16.2(0.31%) 6.55(12.40%)
2 5.19 0(0.00%) 0.0061(0.00%) 3.33(0.64%) 17.4(3.35%) 11.1(2.14%) 151(2.91%) 20.9(0.40%) 4.91(9.50%)
3 5.15 2.3(0.00%) 0.016(0.00%) 1.21(0.23%) 3.26(0.63%) 16.4(3.17%) 69.1(1.34%) 15.4(0.30%) 2.93(5.70%)
4 6.35 0.55(0.00%) 0.024(0.00%) 2.56(0.40%) 12.7(1.99%) 42.2(6.64%) 65.5(1.03%) 69.5(1.09%) 7.09(11.20%)
5 4.99 0(0.00%) 342(6.85%) 1.31(0.26%) 10.6(2.13%) 6.47(1.30%) 88.3(1.77%) 44.3(0.89%) 6.58(13.20%)
6 2.59 0(0.00%) 211(8.15%) 0.81(0.31%) 6.63(2.56%) 0.85(0.33%) 6.93(0.27%) 37.4(1.44%) 3.38(13.10%)
7 10.7 797(0.74%) 867(8.08%) 4.88(0.46%) 10(0.93%) 22.9(2.13%) 131(1.22%) 43.3(0.40%) 15(14.00%)
8 2.11 9.8(0.05%) 1.7(0.08%) 0.1(0.05%) 0.39(0.18%) 14.9(7.05%) 0.011(0.00%) 2.67(0.13%) 1.59(7.50%)
9 1.66 186(1.12%) 0.097(0.01%) 3.71(2.24%) 1.97(1.19%) 29.7(17.90%) 90.5(5.47%) 2.46(0.15%) 4.65(28.10%)
10 2.46 378(1.54%) 0.063(0.00%) 3.63(1.48%) 5.25(2.14%) 21.2(8.62%) 74.1(3.01%) 5.66(0.23%) 4.18(17.00%)

* Values are expressed as absolute counts and percentage of total bacteria (in parentheses), Universal primer 1 was used for enumeration of total bacteria and species-specific primers for A. actinomycetemcomitans (Aa), P. gingivalis (Pg), T. denticola (Td), T. forsythia (Tf), F. nucleatum (Fn), P. intermedia (Pi) and C. rectus (Cr).