Table 1: Percent Recovery of [>14C]-VNP40101M from Incubations with Rat, Dog, Monkey and Human Liver Microsomes (1 mgProtein/mL) in the Presence and Absence of NADPH

Species Cofactor (NADPH) Incubation Time(min) Supernatant Percent Recovery Pellet Percent Recovery Total Percent Recovery for Supernatant and Pellet
Rat + 0 98.6% 9.0% 107.6%
60 95.1% 11.6% 106.7%
0 99.9% 9.5% 109.4%
60 91.6% 12.2% 103.7%
Dog + 0 99.5% 9.3% 108.8%
60 93.8% 11.2% 105.0%
0 97.8% 10.1% 107.9%
60 90.8% 11.6% 102.4%
Monkey + 0 98.6% 9.4% 108.0%
60 94.0% 11.2% 105.2%
0 99.6% 9.1% 108.7%
60 91.7% 12.2% 103.9%
Human + 0 97.8% 9.8% 107.6%
60 97.8% 12.0% 109.8%
0 97.2% 9.7% 106.9%
60 92.1% 12.4% 104.5%

+ NADPH present, − NADPH absent. Recovery determined by liquid scintillation counting. Percentages are the mean of duplicate determinations and are rounded to one decimal place.