Table 2: Conversion of [>14C]-VNP40101M (100 µM) to Radioactive Components (C-1, C-2, C-3, C-4 and C-7) by Rat, Dog, Monkey and Human Liver Microsomes (1 mg Protein/mL) in the Presence and Absence of NADPH

Species Cofactor (NADPH) Incubation Time (min) C-1 Formed Per Incubation (pmol)1 C-2 Formed Per Incubation (pmol)1 C-3 Formed Per Incubation (pmol)1 C-4 Formed Per Incubation (pmol)1 1 Mean Substrate Per Incubation (pmol) Total pmol Per Incubation Percent Loss of Substrate (%) Mass Balance
Rat + 0 NC NC NC NC NC 54300 54300 NA 100.0
60 9770 2320 NC 17300 199 20100 49600 63.0 91.4
- 0 NC NC NC NC NC 50700 50700 NA 100.0
60 10600 2370 NC 17000 NC 21000 50900 58.6 100.6
Dog + 0 NC NC NC NC NC 51900 51900 NA 100.0
60 11500 2590 1640 22400 4070 9370 51500 81.9 99.3
- 0 NC NC NC NC NC 53900 53900 NA 100.0
60 11600 2640 861 22200 NC 25400 62700 52.8 116.4
Monkey + 0 NC NC NC NC NC 56800 56800 NA 100.0
60 12200 2290 2250 20800 2430 13700 53600 75.9 94.3
- 0 NC NC NC NC NC 55500 55500 NA 100.0
60 12200 2620 1520 20500 NC 21600 58400 61.1 105.2
Human + 0 NC NC NC NC NC 62400 62400 NA 100.0
60 10200 3100 1410 19200 171 22700 56800 63.7 91.0
- 0 NC NC NC NC NC 56500 56500 NA 100.0
60 10900 2700 1350 21200 NC 23300 59400 58.7 105.3

+ NADPH present, - NADPH absent, NA: Not applicable, NC: Not calculated (represents a zero or negative value beyond the AUC level).
1 Values have been blank-corrected with appropriate zero-time sample. Values are the mean of duplicate determinations. Values are rounded to three significant figures. Percentages are rounded to one decimal place. Mass balance = Total pmol ÷ Total pmol at zero-time x 100%. Lower limit of quantitation was 0.25 µM (2.5 pmol per injection).