Table 3: Percent Conversion of [>14C]-VNP40101M (100 µM) to Radioactive Components (C-1, C-2, C-3, C-4 and C-7) by Rat, Dog, Monkey and Human Liver Microsomes (1 mg Protein/mL) in the Presence and Absence of NADPH

Species Cofactor (NADPH) Incubation Time(min) Percent C-1 Formation (%) Percent C-2 Formation (%) Percent C-3 Formation (%) Percent C-4 Formation (%) Percent C-7 Formation (%) Total Formation (%) Percent Loss of Substrate (%)
Rat + 0 NC NC NC NC NC NC NA
60 18.0 4.3 NC 31.8 0.4 54.4 63.0
- 0 NC NC NC NC NC NC NA
60 20.9 4.7 NC 33.7 NC 59.2 58.6
Dog + 0 NC NC NC NC NC NC NA
60 22.2 5.0 3.2 43.1 7.8 81.2 81.9
- 0 NC NC NC NC NC NC NA
60 21.5 4.9 1.6 41.2 NC 69.2 52.8
Monkey + 0 NC NC NC NC NC NC NA
60 21.4 4.0 4.0 36.5 4.3 70.2 75.9
- 0 NC NC NC NC NC NC NA
60 21.9 4.7 2.7 37.0 NC 66.4 61.1
Human + 0 NC NC NC NC NC NC NA
60 16.4 5.0 2.3 30.8 0.3 54.6 63.7
- 0 NC NC NC NC NC NC NA
60 19.4 4.8 2.4 37.5 NC 64.0 58.7

+ NADPH present, - NADPH absent. Values have been blank-corrected with appropriate zero-time sample. Values are the mean of duplicate determinations. Values and percentages are rounded to one decimal place. NA: Not applicable, NC: Not calculated (represents a zero or negative value beyond the AUC level). Percent Formation (%) = (Formation per Incubation (pmol) ÷ Mean Substrate per Incubation (pmol) at zero-time) x 100%. Total formation (%) = Sum of Percent Formation values.