Table 6: Analysis of the Correlation Between the Rate of Formation of C-7 from [14C]-VNP40101M (25 µM) and Marker CYP and FMO Activities in a Bank of Human Liver Microsomes (n = 10)

Activity/Enzyme C-7 Correlation Coefficient
Phenacetin O-dealkylase CYP1A2 -0.0698
Coumarin 7-hydroxylase CYP2A6 0.6781
Bupropion hydroxylase CYP2B6 0.923
Paclitaxel 6α -hydroxylase CYP2C8 0.452
Diclofenac 4β-hydroxylase CYP2C9 -0.343
S-Mephenytoin 4β-hydroxylase CYP2C19 -0.0231
Dextromethorphan O-demethylase CYP2D6 0.6911
Chlorzoxazone 6-hydroxylase CYP2E1 -0.442
Testosterone 6β-hydroxylase CYP3A4/5 0.572
Midazolam 1β-hydroxylase CYP3A4/5 0.461
Lauric acid 12-hydroxylase CYP4A11 0.137
Benzydamine N-oxidation FMO -0.113

1 The moderate correlation observed may be artifact due to the enzyme to enzyme correlation with CYP2B6 activity among the individual microsomal samples (i.e., r = 0.598 and r = 0.401). Samples from 16 individuals were evaluated but only data from 10 individuals were used in the data processing.