Cells from one CLL patient were cultured at 2 × 10⁶/ml in the presence or not of Resiquimod 1µM, together or not with BAFF-R-Fc (50µg/ml) or BCMA-Fc (50 µg/ml). After 48 hours of incubation, 20 000 cells were collected and the percentage of enrichment in cytoplasmic nucleosomes was estimated from triplicate samples by a specific ELISA, as described in Materials and Methods. Statistical significances in comparison with control (unstimulated) cells, taken as 100%, were estimated with the modified Student’s t test for a small number of samples. Data are from one patient out of three that gave comparable results.