Fig. (3) Increase in ARPE19 cell senescence in response to increased levels of hydrogen peroxide concentration (oxidative stress). Cells with and without hydrogen peroxide treatments were incubated with Beta Galactosidase stain for 24 hours. Blue, positive cells vs non-stained cells were then counted to calculate the percent expression and cell senescence (mean ± SEM, n=3). One-way ANOVA shows a significant treatment effect (F=614; p ≤0.001). This experiment was repeated twice with similar results.