Fig. (4) Western blot analysis of total protein extracts of control and patients P4 and P6 that were submitted to a lectin-enrichment protocol using wheat germ lectin beads. A: In the control tissue sample, WGL-enriched fractions contain both the α-DG and β-DG subunits. B: WGL-enriched fractions from patients P4 and P6, analysed with antibodies against β-DG. β-DG, that is present in the total protein extracts of both patients, is not enriched upon WGL incubation, confirming the presence of altered glycosylation of α-DG.