Table 2: Venous blood analyte values from collected Titmice based on age, sex, and midazolam administration.
| Means, standard error, and sample size of untransformed values reported for reference purposes. Probability values from means' significance testing provided with two degrees of freedom. Significant values (α ≤ 0.05) are shown in bold. |
| a. Age |
| Analyte |
Units |
n |
Juvenile |
n |
Adult |
p |
| pHNT
|
at 37°C |
21 |
7.56 ±0.01 |
29 |
7.59 ±0.01 |
0.09 |
| pO2
|
mmHg |
21 |
34.5 ±1.1 |
30 |
35.2 ±0.8 |
0.61 |
| sO2
|
% |
21 |
75 ±2 |
29 |
78 ±1 |
0.13 |
| pCO2
|
mmHg |
21 |
28.7 ±1.2 |
29 |
28.2 ±1.1 |
0.75 |
| HCO3
|
mmol L-1
|
21 |
25.0 ±0.9 |
29 |
27.0 ±0.8 |
0.09 |
| tCO2NT
|
mmol L-1
|
21 |
26.1 ±0.8 |
29 |
27.8 ±0.8 |
0.16 |
| BEecf |
mmol L-1
|
21 |
3.2 ±0.9 |
29 |
5.3 ±0.9 |
0.10 |
| LactateNT
|
mmol L-1
|
16 |
3.8 ±0.3 |
26 |
3.8 ±0.2 |
0.98 |
| Glucose |
mg dL-1
|
20 |
339.4 ±13.5 |
28 |
322.3 ±12.2 |
0.36 |
| Na+
|
mmol L-1
|
20 |
158.5 ±1.0 |
28 |
158.7 ±0.7 |
0.80 |
| K+
|
mmol L-1
|
20 |
3.7 ±0.1, 20 |
28 |
3.7 ±0.1 |
0.99 |
| Cl-
|
mmol L-1
|
15 |
119.6 ±1.1 |
25 |
119.0 ±0.9 |
0.67 |
| BUNT
|
mg dL-1
|
15 |
1.8 ±0.2 |
25 |
1.6 ±0.2 |
0.61M
|
| PCV |
% |
17 |
58 ±2 |
31 |
58 ±1 |
0.87 |
| HctNT
|
% PCV |
20 |
42 ±1 |
28 |
44 ±1 |
0.07W
|
| HbNT
|
g dL-1
|
20 |
14.0 ±0.2 |
28 |
15.0 ±0.3 |
0.06W
|
| b. Sex |
| Analyte |
Units |
n |
Female |
n |
Male |
p |
| pHNT
|
at 37°C |
18 |
7.60 ±0.01 |
31 |
7.59 ±0.01 |
0.15 |
| pO2
|
mmHg |
18 |
33.6 ±1.0 |
31 |
35.5 ±0.8 |
0.16 |
| sO2
|
% |
18 |
74 ±2 |
31 |
78 ±1 |
0.06 |
| pCO2
|
mmHg |
18 |
28.6 ±1.1 |
31 |
28.9 ±1.1 |
0.95 |
| HCO3
|
mmol L-1
|
18 |
25.5 ±0.8 |
31 |
27.4 ±0.9 |
0.23 |
| tCO2NT
|
mmol L-1
|
18 |
25.8 ±1.0 |
31 |
27.7 ±0.8 |
0.13 |
| BEecf |
mmol L-1
|
18 |
3.0 ±0.8 |
31 |
5.2 ±0.9 |
0.10 |
| LactateNT
|
mmol L-1
|
13 |
3.9 ±0.3 |
28 |
3.8 ±0.2 |
0.77 |
| Glucose |
mg dL-1
|
16 |
349.6 ±15.4 |
31 |
317.4 ±11.1 |
0.10 |
| Na+
|
mmol L-1
|
16 |
157.8 ±1.1 |
31 |
159.1 ±0.6 |
0.25 |
| K+
|
mmol L-1
|
16 |
3.9 ±0.2 |
31 |
3.6 ±0.1 |
0.03 |
| Cl-
|
mmol L-1
|
12 |
120.8 ±1.1 |
27 |
118.7 ±0.8 |
0.14 |
| BUNT
|
mg dL-1
|
12 |
2.4 ±0.4 |
27 |
1.5 ±0.1 |
0.14M
|
| PCV |
% |
15 |
58 ±2 |
32 |
59 ±1 |
0.77 |
| HctNT
|
% PCV |
16 |
42 ±1 |
31 |
44 ±1 |
0.04 |
| HbNT
|
g dL-1
|
16 |
14.3 ±0.2 |
31 |
14.97 ±0.2 |
0.06 |
| c. Midazolam |
| Analyte |
Units |
n |
Not Administered |
n |
Administered |
p |
| pHNT
|
at 37°C |
28 |
7.59 ±0.02 |
22 |
7.56 ±0.01 |
0.14W
|
| pO2
|
mmHg |
28 |
36.4 ±0.8 |
23 |
33.2 ±0.9 |
0.01 |
| sO2
|
% |
28 |
80 ±1 |
22 |
72.9 ±1.7 |
0.00 |
| pCO2
|
mmHg |
30 |
25.8 ±1.1 |
22 |
31.9 ±0.7 |
<0.001W |
| HCO3
|
mmol L-1
|
28 |
24.3 ±0.7 |
22 |
28.5 ±0.8 |
<0.001 |
| tCO2NT
|
mmol L-1
|
28 |
25.3 ±0.7 |
22 |
29.4 ±0.8 |
<0.001 |
| BEecf |
mmol L-1
|
28 |
3.0 ±0.8 |
22 |
6.3 ±1.0 |
0.01 |
| LactateNT
|
mmol L-1
|
19 |
4.8 ±0.2 |
23 |
3.0 ±0.1 |
<0.001 |
| Glucose |
mg dL-1
|
25 |
310.0 ±11.6 |
23 |
350.4 ±12.8 |
0.02 |
| Na+
|
mmol L-1
|
25 |
157.5 ±0.7 |
23 |
159.8 ±0.7 |
0.02 |
| K+
|
mmol L-1
|
25 |
3.8 ±0.1 |
23 |
3.5 ±0.1 |
0.02 |
| Cl-
|
mmol L-1
|
17 |
121.3 ±1.0 |
23 |
117.7 ±0.8 |
0.01 |
| BUNT
|
mg dL-1
|
17 |
2.3 ±0.2 |
23 |
1.3 ±0.2 |
<0.001M |
| PCV |
% |
30 |
58 ±1 |
18 |
58 ±1 |
0.92 |
| HctNT
|
% PCV |
25 |
43 ±1 |
23 |
44.0 ±0.9 |
0.49 |
| HbNT
|
g dL-1
|
25 |
14.6 ±0.2 |
23 |
14.8 ±0.3 |
0.61 |
NVariable not normally distributed, tested with Shapiro-Wilk Goodness of Fit test for normality.
TVariable transformed to normalize data. 1/exp(x): pH, Log(x): tCO2, Lactate, 1/sqrt(x): BUN, 1/x: Hct, Hb
W Means have unequal variance, p-value provided from Welch's 2 sided F-test
MVariable unable to reach normal distribution, Mann-Whitney ranked score test performed (Mean scores and Z probability provided)
Abbreviations: pO2: partial pressure oxygen, sO2: saturated oxygen, pCO2: partial pressure carbon dioxide, HCO3: bicarbonate, tCO2: total carbon dioxide, BEecf: base excess, Na+: sodium, K+: potassium, Cl-: chloride, BUN: blood urea nitrogen, Hct: hematocrit, Hb: hemoglobin