Fig. (2) Topology of bootstrap (1000 replications) neighbor-joining tree constructed with Mega6 [37] of 93 Cytb haplotypes for Willow Flycatcher deposited in Genbank (distance measure p-distance), rooted with E. alnorum. Haplotype codes are from [26, 27]. A haplotype followed by an “e” indicates it was found in a population assigned by Paxton et al. [26] to E. t. extimus, and “a” to E. t. adastus, and “e,a” represents a haplotype found in individuals of both subspecies. Although it is not possible to determine the geographic location of other haplotypes (the two MVZ specimens were collected by the author in Minnesota), they were found in breeding individuals taken throughout the range and representing all subspecies [27]. No node received > 60% bootstrap support (those with 50-60% support were groups of two or three haplotypes), showing that none of the four subspecies could be supported irrespective of the geographic locality or subspecies.