Fig. (2) VEGFR-2 and pVEGFR-2 are upregulated in transgenic RB. (A) Western blot analysis was performed in retinal/tumor isolates from LHβTag mice and controls at 4, 8, and 16 weeks of age (n=8 pooled retinas per group). Actin was probed as a loading control. One representative blot is shown of 3 replicates. (B) Graphic representation of one representative experiment showing quantitative changes in VEGFR-2 and pVEGFR-2 levels (in relative units) from Western blot analysis.Animal age (weeks) and tumor status (+ for positive or – for negative tumor burden) is indicated on the x-axis.