Fig. (3) A population of Müller glia from the adult human retina express neural stem cell characteristics in vitro. (A) Cells cultured in the absence of extracellular matrix and growth factors display a characteristic glial morphology. In contrast, cells cultured in the presence of extracellular matrix and FGF2 for 5 days acquire a neural morphology with characteristic axon-like processes (red arrows). (B) Müller stem cells in culture express the retinal progenitor markers CHX10, SOX2 and intermediate filament nestin.