Fig. (4) Induction of Treg cells in the draining lymph nodes from DO11.10TcR-Tg mice (C-) exposed to OVA for 6 h (C+) and treated for 72 h with placebo (P) or 0.02% Levocetirizine ophthalmic solution for 72 h, as determined by flow cytometry of lymphocytes in R1 as in the panel to the left. From these cells we gated the CD4+/CD25+ cells (R2), and analysed the Foxp3+cells from these last gated cells (R3). *p<0.05 vs C+, ap<0.05 vs placebo, Student’s t-test (n = 6).